Year: 2008
Type: article
Abstract: In Search For New p53 Regulated Genes.The p53 tumor suppressor protein has the ability to transactivate its target genes whose gene products are involved in carrying out cell cycle arrest, apoptosis, DNA repair, and senescence.Here, I report that two genes may be p53 regulated.Utilizing a microarray method to search for novel p53 target genes, I was able to identify a possible transcriptional target of p53 being solute carrier family 1a1 (SLC1a1).Along with that finding I also identified an E2F-target gene, minichromosome maintenance 10 (MCM10), as being p53 regulated.Gene expression profiling of MCF7 breast cancer cells treated with RNAi targeting Hdm2 and HdmX in order to reactivate p53 led to increased SLC1a1 transcript levels.DNA damage experiments in several cell lines and along with a p53 overexpression experiment established that p53 activation unexpectedly lead to no transcriptional increase in SLC1a1 expression.The results suggest that SLC1a1 is not a transcriptional target of p53 and may have been a false positive result from the microarray experiment.iii
Author: Meldrick Daniel Mpagi
Institution: Wright State University
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2530380771