Influence of Basal Medium and Hormones on Callus Induction and its Organogenesis from In Vitro Culture of Rose Leaf Segments
Year: 2004
Type: article
Abstract: Influence of culture media and hormone concentrations on plant regeneration from rose ( Rosa hybrida L. cv. Raktagandha) leaf segments were investigated. Leaves were excised from healthy, well-grown and mature plants. Leaf segments (4-5 mm long) were sectioned and cultured on Murashige and Skoog (1962) medium containing different concentration of growth hormones. Callus formation was most prolific (97.09%) on MS medium containing MS basal salts + 0.5 mg·L -1 BAP + 2.0 mg·L -1 2,4-D. maximum (56.67%) organogenesis or shoot differentiation was achieved on MS modified medium supplemented with 1.0 mg L-1 BAP + 0.1 mg·L -1 NAA + 10.0 mg·L -1 Adenine Sulphate. The highest percentage (93.73%) of in-vitro rooting was observed in half-strength MS basal medium containing 0.5 mg·L -1 IBA. Rooted plants were transferred in to sterilized potting mixture and grown in a greenhouse.
Source: HortScience
Authors: Rajendra Maurya*, Nathu Ram Godara*, Ram C. Yadav*
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $3,000
OpenAlex ID: W2530454638