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Inter- and Intra-Strain Variability of Tandem Repeats in Mycoplasma Pneumoniae Based on Next-Generation Sequencing Data

Year: 2016
Type: article
Abstract: AIM: To characterize inter- and intra-strain variability of variable-number tandem repeats (VNTRs) in Mycoplasma pneumoniae to determine the optimal multilocus VNTR analysis scheme for improved strain typing. METHODS: Whole genome assemblies and next-generation sequencing data from diverse M. pneumoniae isolates were used to characterize VNTRs and their variability, and to compare the strain discriminability of new VNTR and existing markers. RESULTS: We identified 13 VNTRs including five reported previously. These VNTRs displayed different levels of inter- and intra-strain copy number variations. All new markers showed similar or higher discriminability compared with existing VNTR markers and the P1 typing system. CONCLUSION: Our study provides novel insights into VNTR variations and potential new multilocus VNTR analysis schemes for improved genotyping of M. pneumoniae.
DOI: 10.2217/fmb-2016-0111
Authors: Jing Zhang, Xiaohong Song, Marella J Ma, Li Xiao, Tsuyoshi Kenri, Hongmei Sun, Travis Ptacek, Shaoli Li, Ken B Waites, T Prescott Atkinson, Keigo Shibayama, Kevin Dybvig, Yanmei Feng
Institutions: National Institutes of Health, National Institutes of Health Clinical Center, Chongqing Medical University, Sichuan University, University of Alabama at Birmingham, National Institute of Infectious Diseases, Capital Institute of Pediatrics
Language: English
Subfield: Microbiology
Open Access status: green
Funders: Chongqing Medical University, China Scholarship Council, National Institutes of Health, Japan Society for the Promotion of Science, National Institute of Allergy and Infectious Diseases, NIH Clinical Center, National Center for Advancing Translational Sciences
OpenAlex ID: W2531552252
FWCI: 2.043
Cites: 51
Cited by: 8
Related to: 10