Year: 2009
Type: article
Abstract: Interferon stimulated genes (ISGs) are induced in diverse situations.These include infection by virus, bacteria and parasites as well as other biological processes involved in autoimmunity, cancer and even the female menstrual cycle.Of the hundreds of genes that are upregulated, ISG56 is consistently one of the, if not the, strongest induced gene.The protein which it encodes, p56, has been shown to mediate antiviral activity against human papillomavirus and hepatitis C virus by inhibiting translation.To determine the physiological functions of this antiviral activity as well as other processes in which ISG56 has been implicated we sought to disrupt the mouse ISG56 gene by making a conditional knockout.Unfortunately, problems were encountered in the generation of an embryonic stem cell containing a correctly integrated targeting construct.Possible reasons for this failure, methods to improve the chances for targeting success and the predicted phenotype of the ISG56 knockout are discussed.
Author: Lenette L. Lu
Institution: Case Western Reserve University
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2532956647