Comparison of gating dynamics of different IP 3 R channels with immune algorithm searching for channel parameter distributions
Year: 2016
Type: article
Abstract: The gating properties of the inositol 1, 4, 5-trisphosphate (IP 3 ) receptor (IP 3 R) are determined by the binding and unbinding capability of Ca 2+ ions and IP 3 messengers. With the patch clamp experiments, the stationary properties have been discussed for Xenopus oocyte type-1 IP 3 R (Oo-IP 3 R1), type-3 IP 3 R (Oo-IP 3 R3) and Spodoptera frugiperda IP 3 R (Sf-IP 3 R). In this paper, in order to provide insights about the relation between the observed gating characteristics and the gating parameters in different IP 3 Rs, we apply the immune algorithm to fit the parameters of a modified DeYoung–Keizer model. By comparing the fitting parameter distributions of three IP 3 Rs, we suggest that the three types of IP 3 Rs have the similar open sensitivity in responding to IP 3 . The Oo-IP 3 R3 channel is easy to open in responding to low Ca 2+ concentration, while Sf-IP 3 R channel is easily inhibited in responding to high Ca 2+ concentration. We also show that the IP 3 binding rate is not a sensitive parameter for stationary gating dynamics for three IP 3 Rs, but the inhibitory Ca 2+ binding/unbinding rates are sensitive parameters for gating dynamics for both Oo-IP 3 R1 and Oo-IP 3 R3 channels. Such differences may be important in generating the spatially and temporally complex Ca 2+ oscillations in cells. Our study also demonstrates that the immune algorithm can be applied for model parameter searching in biological systems.
Source: Physical Biology
Authors: Xiuhong Cai, Xiang Li, Hong Qi, Fang Wei, Jianyong Chen, Jianwei Shuai
Institutions: Xiamen University, Shanxi University, Shenzhen University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Awards: G8195903831, 胞质钙信号调控细胞凋亡的动力学机制研究
OpenAlex ID: W2538468432