Work

Facile Coating Strategy to Functionalize Inorganic Nanoparticles for Biosensing

Year: 2016

Type: article

Abstract: The use of inorganic nanoparticles (NPs) for biosensing requires that they exhibit high colloidal stability under various physiological conditions. Here, we report on a general approach to render hydrophobic NPs into hydrophilic ones that are ready for bioconjugation. The method uses peglyated polymers conjugated with multiple dopamines, which results in multidentate coordination. As proof-of-concept, we applied the coating to stabilize ferrite and lanthanide NPs synthesized by thermal decomposition. Both polymer-coated NPs showed excellent water solubility and were stable at high salt concentrations under physiological conditions. We used these NPs as molecular-sensing agents to detect exosomes and bacterial nucleic acids.

Source: Bioconjugate Chemistry

DOI: 10.1021/acs.bioconjchem.6b00524

Authors: Yong Il Park, Eunha Kim, Chen-Han Huang, Ki Soo Park, Cesar M. Castro, Hakho Lee, Ralph Weissleder

Institutions: Harvard University, Massachusetts General Hospital, Center for Systems Biology

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering, Congressionally Directed Medical Research Programs

Awards: G1098359285, Multiplexed analysis of exosomes in cancer nano therapy, Novel Clickdyes for Biomedical Sensing, G2066243351, G2528638505, The MIT-Harvard Center of Cancer Nanotechnology Excelle*, MAGNETIC NANOSENSORS FOR BIOMEDICAL ANALYSES OF MICROVESICLES, Chip NMR biosensor for molecular analysis of cells, Chip-Based Magnetic Imager for Molecular Profiling of Ovarian Cancer Cells, Multiplexed exosome analyses with DNA barcoding, TRAINING GRANT IN MOLECUCLAR IMAGING RESEARCH AND CANCER SYSTEMS ANALYSIS, G947370982

OpenAlex ID: W2540086619

FWCI: 1.559

Cites: 23

Cited by: 18

Related to: 10