Work

Using electron microscopy to calculate optical properties of biological samples

Year: 2016

Type: article

Abstract: The microscopic structural origins of optical properties in biological media are still not fully understood. Better understanding these origins can serve to improve the utility of existing techniques and facilitate the discovery of other novel techniques. We propose a novel analysis technique using electron microscopy (EM) to calculate optical properties of specific biological structures. This method is demonstrated with images of human epithelial colon cell nuclei. The spectrum of anisotropy factor g, the phase function and the shape factor D of the nuclei are calculated. The results show strong agreement with an independent study. This method provides a new way to extract the true phase function of biological samples and provides an independent validation for optical property measurement techniques.

Source: Biomedical Optics Express

DOI: 10.1364/boe.7.004749

Authors: Wenli Wu, Andrew J. Radosevich, Adam Eshein, The-Quyen Nguyen, Ji Yi, Lusik Cherkezyan, Hemant K. Roy, Igal Szleifer, Vadim Backman

Institutions: Northwestern University, Boston University, Boston Medical Center, Chemistry of Life Processes Institute

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $1,913

Funders: National Science Foundation, National Institutes of Health, National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering, Division of Emerging Frontiers in Research and Innovation

Awards: G1123447905, G1259591088, Spatio-Temporal Organization of Chromatin and Information Transfer in Cancer, G4669176925, EFRI-BioFlex: Miniature, low-cost fiber-optics technology for measurement of tissue structure at sub-diffractional length scales: a platform for cancer screening, G783554857, In-vivo characterization of pancreatic field carcinogenesis using spatially resolved reflectance measurements via a fiber optic probe, G9000108312, G9000261853, The Robert H. Lurie Comprehensive Cancer Center

OpenAlex ID: W2542385118

FWCI: 1.313

Cites: 54

Cited by: 7

Related to: 10