Year: 2009
Type: article
Abstract: Ischemic Stroke in Type 2 Diabetic Mice: Deregulation of SDF-1a/CXCR4 axis 1 . 1 Type 2 diabetes mellitus is a major risk factor for ischemic stroke.Also diabetes is associated with poor outcome after stroke.Underlying mechanisms are however not fully understood.Alteration in the expression of the SDF-1a/CXCR4 axis, which is important for ischemic tissue repair, can be a probable cause.In this study, we have determined the expression of SDF-1a/CXCR4 in the brains of type II diabetic mice at basal and in response to ischemic stroke and have investigated a method for overexpression of SDF-1a in the brains of the diabetic mice.Adult male C57BLKS/J mice (db/db) of age 8 weeks were used as the murine model for type II diabetes and their age matched lean littermates served as controls (db/+).Microvascular density was first determined in the cerebral cortex of db/db diabetic mice by immunohistochemical analysis.Focal cerebral ischemia was induced by middle cerebral artery occlusion surgery (MCAO) in type 2 diabetic db/db mice and their controls.48 hours after surgery, volume of ischemic damage was determined by TTC staining.The expression of SDF-1a and CXCR4 in the ischemic and non ischemic sides of brains of both the groups were determined using western blot and real time RT PCR.The db/db diabetic mice were injected with the vi Double Immunohistochemistry after Microinjection . . . . . . . . . . . . . . . . . . . . . . .
Author: Avik Das
Institution: Wright State University
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2542737375