Year: 2002
Type: article
Abstract: A total of two hundred samples from dialysed neuraminidase of Newcastle disease virus (NDV) Kudu 113 strain were used for the experiment.Fifty samples each were used for various concentrations of paranitrophenyl oxamic acid (PNPO), salicyl oxamic acid (SOA), silver nitrate (AgNO 3 ) and ethylene diamine tetra acetic acid (EDTA), to test their inhibition effects on the neuraminidase activity of NDV Kudu 113 strain in vitro, using thiobarbituric acid assay method.Of these inhibitors, AgNO 3 had the highest inhibition effect on the neuraminidase activity of NDV Kudu 113 strain.Silver nitrate reduced the activity of this enzyme from 140.24 µMol/min for enzyme without AgNO 3 to 32.36 µMol/min when AgNO 3 was added to the enzyme at a concentration of 0.47 M. Paranitrophenyl oxamic acid had the least inhibition effect on the neuraminidase activity of NDV Kudu 113 strain at a concentration of 0.12 M.This inhibitor reduced the activity of the enzyme from 97.08 µMol/min for enzyme without PNPO to 91.70 µMol/min when PNPO was added to the enzyme at a concentration of 0.12 M. Ethylene diamine tetra acetic acid and SOA also greatly inhibited the activity of this enzyme.Salicyl oxamic acid reduced the activity of NDV Kudu 113 strain neuraminidase from 140.24 µMol/min for enzyme without SOA to 59.33 µMol/min when SOA was added at a concentration of 0.47 M. Similarly, EDTA reduced the activity of this enzyme from 124.06 µMol/min for Vet.arhiv 72 (4), [185][186][187][188][189][190][191][192][193][194] 2002 S. B. Oladele et al.: Effect of some inhibitors on neuraminidase of Newcastle disease virus Kudu 113 strain enzyme without EDTA to 75.51 µMol/min when EDTA was added to the enzyme at a concentration of 0.35 M. In all the inhibitors examined, it was observed that their effects on the activity of NDV Kudu 113 strain neuraminidase increased as the concentrations of the inhibitors increased.It was concluded that PNPO, SOA, EDTA and AgNO 3 inhibited the neuraminidase activity of NDV Kudu 113 strain in vitro.Therefore, further studies are needed to see if these inhibitors could be of use, after proper clinical trials, as potential agents for reducing Newcastle disease (ND) infections in poultry.
Institution: Ahmadu Bello University
Language: English
Topic: Virology and Viral Diseases
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2549517819