Prevalence of colonization and mitochondrial large subunit rRNA mutation of Pneumocystis jiroveci among Iranian children.
Year: 2016
Type: article
Study design: Observational Study
Abstract: BACKGROUND AND OBJECTIVES: in children without any respiratory syndrome and survey the distribution of different mitochondrial large subunit, ribosomal ribonucleic acid (mtLSU- rRNA) genotypes. MATERIALS AND METHODS: colonization was confirmed by immunofluorescence staining, nested PCR and sequencing. Genotypic characterization at the mitochondrial large subunit rRNA gene was performed by direct sequencing. RESULTS: colonization was 3.5% (6 samples). The results of sequencing revealed the two polymorphisms; 85/A; 248/C in 3 cases, and 85/T; 248/C in other cases. One sample also showed a mutation replacement at position 258 (T-to-C change), which was not reported previously. CONCLUSION: Colonized person as an environmental reservoir might play an important role in the progression of infection in immunocompromised patients. Diagnosis of the reservoir and genotyping can be essential for the prevention of nosocomial infections.
Source: PubMed
Authors: Parisa Badiee, Ali Rezapour, Amin Abbasian, Hamid Reza Foroutan, Hadis Jafarian
Institution: Shiraz University of Medical Sciences
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2550570406