Influence of Diet on Population Growth and Allergen Production in Cultured House Dust Mites - Dermatophagoides Farinae and Dermatophagoides Pteronyssinus
Year: 2009
Type: article
Abstract: Dermatophagoides farinae and Dermatophagoides pteronyssinus culture extracts are used for skin testing, immunotherapy and research studies. There is a need to develop an effective culture method so that standardized extracts and allergens can be produced. The purpose of this study was to examine if different diets effect mite population growth and the amount of allergen produced in cultures. Dermatophagoides farinae and Dermatophagoides pteronyssinus were cultured on diets A and B. The density of mites, life stage composition and allergen concentration was determined at two week intervals. Enzyme-linked immune sorbent assays (ELISA) were used to assess group 1 and group 2 allergen concentrations. This study showed that diet is an important factor for culturing of mites. Diet B was found to be better suited to culture Dermatophagoides farinae whereas diet A was better for culturing of Dermatophagoides pteronyssinus. Diet A cultures of Dermatophagoides pteronyssinus produced greater concentrations of group 2 allergen than diet B.
Author: Swetha Avula Poola
Institution: Wright State University
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2551310957