Year: 1990
Type: article
Abstract: Mung bean (Vigna radiata (L.) R. Wilcz.) cuttings are used in rooting bioassays, and nonexperimental variability must be rigorously controlled to obtain meaningful results. This study was conducted to document bacterial disease problems of mung bean and identify the causal organisms. `Berken' seeds were surfaced sterilized and aerated 24 hr before sowing. Nine-day-old seedlings were used in rooting bioassays. Up to 10% of the seedlings and 17% of the cuttings had collapsed stems or wilted leaves. A white and two yellow (Y1 and Y2) bacteria were isolated from diseased cuttings and used in subsequent pathogenicity tests. The Y2 isolate was nonpathogenic. Stems of healthy mung beans inoculated with the white isolate turned brown and collapsed 2 days after inoculation, whereas leaves of plants inoculated with the yellow isolate wilted after 7 days. Standard biochemical and physiological tests revealed that the white isolate was Pseudomonas syringue pv. syringae van Hall and the yellow isolate was Curtobacterium flaccumfaciens subsp. flaccumfaciens (Hedges) Collins and Jones. This research is the first report of a disease in mung bean caused by P.s. pv. syringae. These results demonstrate the need or disease-free seeds being used in bioassays since both pathogens were seed-borne.
Source: HortScience
Authors: Robert R. Tripepi, Mary W. George
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2552324531