Year: 1994
Type: article
Abstract: DNA was extracted from leaves of various Malus genotypes and used to screen synthetic decamer oligonucleotide primers. Samples from the following two groups were bulked: 1) seven scab-susceptible apple cultivars, and 2) 15 scab-resistant apple genotypes derived by introgressive hybridization from the previous group of cultivars. A third sample consisted of DNA extracted from Malus floribunda Sieb. clone 821, the original source of apple scab resistance for all genotypes in the second group. A total of 59 primers from kits A, L, and R (Operon Technologies) were screened. Amplified fragments were obtained for 93% of the primers tested, while random amplified polymorphic DNA (RAPD) fragments were detected among samples for 76% of the primers. One primer, A15, amplified a unique band in both M. floribunda clone 821 and the bulked scab-resistant sample. This RAPD marker, designated OA15 900 , identifies an amplified, introgressed fragment that likely corresponds to a region of the genome that may serve as a modifier for the scab resistance gene block V, derived from M. floribunda clone 821.
Source: HortScience
Authors: Richard E. Durham, Schuyler S. Korban
Language: English
Subfield: Genetics
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2553482179