Label free and multiplexed immunosensor chips for detection of macromolecular and cellular biomarkers
Year: 2015
Type: article
Abstract: Biomarker detection represents an important task for many scientific fields such as disease diagnosis, biodefense, environmental monitoring and biological research.In this research, micro resistive pulse immunoaggregation sensors were studied to quantitatively detect macromolecular and cellular biomarker concentrations.First, to prove the concept of the immunoaggregation assay and resistive pulse sensing, goat anti-rabbit IgG, used as a model biomarker, was detected by the micro resistive pulse immunoaggregation sensor.A detection range from 16.0 to 160 ng/mL was achieved.The human ferritin in 10% fetal bovine serum was then detected to prove the device's performance in a complex media.A detection range from 0.104 to 208 ng/mL was achieved.In addition, the studies showed that the detection range can be shifted to lower and higher concentrations by decreasing and increasing MP concentrations, respectively.Second, the multiplexed immunoaggregation assay was also studied in this research to achieve multiple biomarker detection in a single test.A mixture consisting of multiple types of MPs functionalized by different antibody with different sizes and magnetic properties was used for testing the multiplexed assay.2.00 µm non-magnetic MPs coated with rabbit anti-mouse IgG antibody and 2.80 µm magnetic MPs coated with goat antihuman ferritin antibody were used to detect the concentration of mouse anti-rabbit IgG and CHAPTER I
Author: Yu Han
Institution: University of Akron
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2555136953