Dynamic Microenvironment Induces Phenotypic Plasticity of Esophageal Cancer Cells Under Flow
Year: 2016
Type: article
Abstract: Cancer microenvironment is a remarkably heterogeneous composition of cellular and non-cellular components, regulated by both external and intrinsic physical and chemical stimuli. Physical alterations driven by increased proliferation of neoplastic cells and angiogenesis in the cancer microenvironment result in the exposure of the cancer cells to elevated levels of flow-based shear stress. We developed a dynamic microfluidic cell culture platform utilizing eshopagael cancer cells as model cells to investigate the phenotypic changes of cancer cells upon exposure to fluid shear stress. We report the epithelial to hybrid epithelial/mesenchymal transition as a result of decreasing E-Cadherin and increasing N-Cadherin and vimentin expressions, higher clonogenicity and ALDH positive expression of cancer cells cultured in a dynamic microfluidic chip under laminar flow compared to the static culture condition. We also sought regulation of chemotherapeutics in cancer microenvironment towards phenotypic control of cancer cells. Such in vitro microfluidic system could potentially be used to monitor how the interstitial fluid dynamics affect cancer microenvironment and plasticity on a simple, highly controllable and inexpensive bioengineered platform.
Source: Scientific Reports
DOI: 10.1038/srep38221
Language: English
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,690
Funders: National Science Foundation, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of Neurological Disorders and Stroke, National Institute of Biomedical Imaging and Bioengineering, National Institute of Dental and Craniofacial Research
Awards: G1614795888, Stanford Neuroscience Research Cores for Gene Vectors, Microscopy, and Behaviors, G1932930224, G1991958910, G3330864316, G3495971452, G3739355248, CAREER: Non-invasive fields for directed 3D microgel assembly for tissue engineering, G4192305720, Collaborative Research: EAGER: Biomanufacturing: Bioengineering of 3-dimensional brain surrogate tissue models, Magnetic Cellular Assembly and Microfluidic Conditioning for Generation of Functi, G589803372, Center for Cancer Nanotechnology Excellence for Translational Diagnostics (CCNE-TD), G6671297155, Minimizing the role of cryoprotectant toxicity for cryopreservation, G78733395, G9000042032, G9000045412, G9000053670, G9000138777, G910680712
OpenAlex ID: W2559342398