Patchiness of Ciliate Communities Sampled at Varying Spatial Scales along the New England Shelf
Year: 2016
Type: article
Abstract: Although protists (microbial eukaryotes) provide an important link between bacteria and Metazoa in food webs, we do not yet have a clear understanding of the spatial scales on which protist diversity varies. Here, we use a combination of DNA fingerprinting (denaturant gradient gel electrophoresis or DGGE) and high-throughput sequencing (HTS) to assess the ciliate community in the class Spirotrichea at varying scales of 1-3 km sampled in three locations separated by at least 25 km-offshore, midshelf and inshore-along the New England shelf. Analyses of both abundant community (DGGE) and the total community (HTS) members reveal that: 1) ciliate communities are patchily distributed inshore (i.e. the middle station of a transect is distinct from its two neighboring stations), whereas communities are more homogeneous among samples within the midshelf and offshore stations; 2) a ciliate closely related to Pelagostrobilidium paraepacrum 'blooms' inshore and; 3) environmental factors may differentially impact the distributions of individual ciliates (i.e. OTUs) rather than the community as a whole as OTUs tend to show distinct biogeographies (e.g. some OTUs are restricted to the offshore locations, some to the surface, etc.). Together, these data show the complexity underlying the spatial distributions of marine protists, and suggest that biogeography may be a property of ciliate species rather than communities.
Source: PLoS ONE
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Life below water
Open Access status: gold
Estimated APC paid: $2,382
Awards: G1214371765, G1460967580, G1604024281, Collaborative research: Diversity and dynamics of planktonic ciliates - what can next-generation sequencing technologies tell us?, G2119709054, G3614316869, G531025577, Collaborative research: Patterns of diversity in planktonic ciliates ? spatio-temporal scales and community assembly in the coastal ocean, Collaborative research: Patterns of diversity in planktonic ciliates: spatio-temporal scales and community assembly in the coastal ocean, G709448157, G7657794787, Acquisition of Instrumentation for a Genomics Facility in Support of the URI Environmental Biotechnology Initiative, Collaborative research: Diversity and dynamics of planktonic ciliates - what can next-generation sequencing technologies tell us?, G837490791, Rhode Island EPSCoR: Catalyzing a Research, Education and Innovation Network, Infrastructure to Advance Life Sciences in the Ocean State
OpenAlex ID: W2559974747