In Vitro Medium-Term Conservation of Several Carnation Accessions through Nutrient Modification
Year: 2016
Type: article
Abstract: Modification of osmotic pressure of in vitro medium-term can be applied to conserve carnation accessions. This successful method inspired the use of nutrient modification technique to have the same function as far as these concerned. The research was then, conducted to evaluate in vitro media for preserving several carnation accessions in low temperature conditions. The research was carried out at Indonesian Ornamental Crops Research Institute (IOCRI) from January 2007 to March 2008. A randomized completely block design with 25 replications was used to accomplish the combination of two factors. The first factor was six commercial carnation accessions, namely cv. Light Pink Candy, Aicardy, Torpedo, Malaga, President, and Rendezvouz. While the second factor dealt with four formulations of conservation media i.e. ½ MS + DMSO 3% + 4% sucrose, modified hyponex + 4% sucrose, modified hyponex (no sucrose) and ½ MS + 4% sucrose (control). The results showed that medium term in vitro conservation for carnation were successfully conducted up to 10 months using in ½ MS + DMSO 3% + 4% sucrose and modified hyponex + 4% sucrose without any significant differences and decreases in viability when transferred in to shoot induction media. In the absent of sucrose, however, the plantlet survivals were only up to 8 months. No significant variation on viability and number of survival plantlet was observed among the carnation accessions in all media tested.
Source: Buletin Plasma Nutfah
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2559983136