Year: 2015
Type: dissertation
Abstract: Hypoxia and centrosome aberrations are both prominent features of prostate cancer and have been shown to be markers of poor clinical prognosis. Hypoxia has been linked to increased genomic instability and aggressive tumor phenotype. Centrosome aberrations have also been directly linked to chromosomal instability. I hypothesized that hypoxia causes centrosome aberrations in prostate cancer cells, as a potential mechanism of hypoxia-induced genomic instability. Analyses of centrosome gene expression in DU14 prostate cancer cells demonstrated that hypoxia causes centrosome gene deregulation. Centrosome structure assays revealed that hypoxia leads to accumulation of centriolar satellites in a cell line and re-oxygenation independent manner. If centrosome aberrations are specific to hypoxic cancer cells, centrosome and mitosis targeting drugs could provide a high therapeutic ratio and better outcome for patients with high hypoxic tumor fraction.
Source: TSpace (University of Toronto)
Author: Daria Taiakina
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2561174159