The Effect of Omega-3 Polyunsaturated Fatty Acids on Growth and microRNA-21 Expression in Estrogen Receptor-Positive Breast Cancer Cells (MCF-7)
Year: 2015
Type: dissertation
Abstract: Targeted, effective and safe therapies for breast cancer are lacking and many are turning towards complementary therapies, such as FS oil (FSO) and fish oil (FO). Interest surrounding the role of microRNA (miR) in breast cancer has piqued over the last decade, and the interaction between miR and dietary factors is emerging. This thesis examined the role of α-linolenic acid (ALA) alone or combined with eicosapentaenoic acid (EPA) and docoashexaenoic acid (DHA) at ratios seen in the serum post-FSO consumption on cell growth, miR-21 expression and molecular targets of miR-21 in estrogen receptor-positive breast cancer (MCF-7) cells. A reduction in cell growth and miR-21 expression was observed following fatty acid treatment of varying incubation times. Bcl-2 gene is one molecular target of miR-21 that was reduced by the fatty acid treatment. In conclusion, ALA alone or combined with EPA and DHA can reduce cell growth and miR-21 expression.
Source: TSpace (University of Toronto)
Author: Lauren LeMay‐Nedjelski
Language: English
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2562272280