Assessment of the expression of 5-HT receptors in thehippocampus of patients with temporal lobe epilepsy with hippocampal sclerosis
Year: 2017
Type: dissertation
Study design: Observational Study
Abstract: Temporal lobe epilepsy with hippocampal sclerosis (TLE-HS) is the most common type of pharmaco-resistant epilepsy. It is characterised by recurrent focal onset seizure that affects the medial part of the temporal lobe, in particular, the hippocampus. Patients with TLE-HS suffer from typical aura symptoms, especially epigastric aura, in addition to psychological symptoms which include dysmnestic symptoms such as memory impairment and fear. As a result of the important role of the serotonergic system in human brain physiological and pathological functions and the crucial need to identify a novel target to control seizure among this type of patient, some serotonergic excitatory (5-HT\\(_2\\)\\(_A\\), 5-HT\\(_3\\)A, 5-HT\\(_4\\) and 5-HT\\(_7\\)) and inhibitory (5-HT\\(_1\\)\\(_A\\)) receptors were selected to study using immunohistochemistry in the human TLE-HS hippocampus, in addition to the purinergic receptors (P2x\\(_7\\)) and the orphan G-protein (GPR61). The results indicate variable degree of upregulation of 5-HT\\(_3\\)A, 5-HT\\(_1\\)\\(_A\\), 5-HT\\(_4\\), GPR61 and P2x\\(_7\\) receptors in the sclerotic hippocampi of TLE-HS patients. Subsequent studies focused on the 5-HT\\(_3\\)A receptors expression; the overexpression of this excitatory receptor was established using immunohistochemistry, SDS-PAGE western blot, receptor autoradiography and radioligand binding assays that give comparable results. The upregulation of the excitatory 5-HT\\(_3\\)A receptor in the excitatory neurons of hippocampus may contribute to the neuronal hyperexcitability evident among TLE-HS patients. This study proposed 5-HT\\(_3\\) receptors as a novel target to antagonise to control seizure in TLE-HS patients and improve their quality of life.
Author: Hatun Alomar
Language: English
Subfield: Clinical Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2562442437