Year: 2014
Type: article
Abstract: We previously reported that posttransplant alloantibody production in CD8-deficient hosts is IL-4+ CD4+ T cell-dependent and IgG1 isotype-dominant. The current studies investigated the hypothesis that IL-4-producing natural killer T cells (NKT cells) contribute to maximal alloantibody production. To investigate this, alloantibody levels were examined in CD8-deficient WT, CD1d KO and Jα18 KO transplant recipients. We found that the magnitude of IgG1 alloantibody production was critically dependent on the presence of type I NKT cells, which are activated by day 1 posttransplant. Unexpectedly, type I NKT cell contribution to enhanced IgG1 alloantibody levels was interferon-γ-dependent and IL-4-independent. Cognate interactions between type I NKT and B cells alone do not stimulate alloantibody production. Instead, NKT cells appear to enhance maturation of IL-4+ CD4+ T cells. To our knowledge, this is the first report to substantiate a critical role for type I NKT cells in enhancing in vivo antibody production in response to endogenous antigenic stimuli.
DOI: 10.1111/ajt.12922
Authors: J.M. Zimmerer, P. Swamy, P.B. Sanghavi, C.L. Wright, M. Abdel-Rasoul, S.M. Elzein, R.R. Brutkiewicz, G.L. Bumgardner
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,300
Awards: Novel Mechanisms Regulating Posttransplant Humoral Alloimmunity, CDI Molecules & NKT Cells in Host Defense, G481147175, G5229421098, CD8+ T cell regulation of alloantibody production following transplantation, CD1 Molecules & NKT Cells in Host Defense, G8355548385, Antigen-Presenting Function of CD1 Molecules, G9000301091, G9000318135, Indiana University Melvin and Bren Simon Comprehensive Cancer Center Support Grant
OpenAlex ID: W1835415418