Work

705. Enhancement of CAR Expression of piggyBac Transposon-Engineered T Cells by Stimulation with Viral Antigens

Year: 2016

Type: article

Abstract: Introduction We previously showed that the piggyBac transposon system is a promising genetic tool for stable, non-viral gene engineering of primary human T cells. Our initial culture strategy for expanding piggyBac-modified CD19 CAR-T cells required magnetic cell isolation due to low CAR expression and 21 days of culture and our CAR included an IgG1-Fc spacer that may limit CAR-T cell expansion in vivo. We have now improved these process and construct to avoid magnetic cell isolation, shorten the culture period, and increase in vivo efficacy.

Source: Molecular Therapy

DOI: 10.1016/s1525-0016(16)33513-4