p53 mutational analysis in laryngeal squamous cell carcinoma, results of full-length gene sequencing
Year: 1999
Type: dissertation
Study design: Observational Study
Abstract: In this study DNA was extracted from 52 paraffin embedded, formalin fixed biopsies from 42 laryngeal squamous cell carcinomas. Exons 2-11 of the p53 gene were individually amplified and sequenced. Standard IHC was performed using the monoclonal antibodies DO7 and EA1 to detect p53 and p2l proteins respectively. 22 mutations were observed in tumours from 20 patients (48%). 5 of these mutations (23%) were outside exons 5-8. Discordance between p53 genotype and protein levels was observed. Similarly, there was no association between p53 status (genotype or IHC) and either p21 protein expression or local recurrence following radiation therapy. These results show that p53 mutations are common in laryngeal carcinomas and that a large proportion occurs outside traditionally examined regions. The lack of correlation with outcome suggests p53 mutations and protein expression are not associated with tumour response to radiation therapy in laryngeal squamous cell carcinoma.
Author: Elizabeth Saunders
Language: English
Topic: Head and Neck Cancer Studies
Subfield: Otorhinolaryngology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2563394076