Work

Development of ultrahigh resolution FTICR mass spectrometry methods for clinical proteomics

Year: 2014

Type: dissertation

Abstract: Fourier transform ion cyclotron resonance (FTICR) MS provides (a) ultrahigh mass resolving power that allows the analysis of large proteins and complex mixtures; (b) ultrahigh mass measurement accuracy and precision that allow a more reliable identification of the detected species; (c) a wide dynamic range that is favourable for the detection of low abundant components. Despite these excellent characteristics, MALDI-FTICR MS has been rarely used for peptide and protein profiling of large cohorts of samples. In this thesis, the development and application of novel MALDI-FTICR-MS methods for high-throughput analysis of human serum peptides and proteins is described. Due to the large biological variability of human serum, the analysis of a large cohort is needed to obtain reliable statistical results.

Source: Leiden Repository (Leiden University)

Author: Simone Nicolardi

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Leids Universitair Medisch Centrum, Center for Translational Molecular Medicine

OpenAlex ID: W2563526029

Cites:

Cited by: 1

Related to: 10