Year: 2016
Type: article
Abstract: Cancer cells have been increasingly grown in pharmaceutical research to understand tumorigenesis and develop new therapeutic drugs. Currently, cells are typically grown using two-dimensional (2-D) cell culture approaches, where the native tumor microenvironment is difficult to recapitulate. Thus, one of the main obstacles in oncology is the lack of proper infection models that recount main features present in tumors. In recent years, microtechnology-based platforms have been employed to generate three-dimensional (3-D) models that better mimic the native microenvironment in cell culture. Here, we present an innovative approach to culture Kaposi's sarcoma-associated herpesvirus (KSHV) infected human B cells in 3-D using a microwell array system. The results demonstrate that the KSHV-infected B cells can be grown up to 15 days in a 3-D culture. Compared with 2-D, cells grown in 3-D had increased numbers of KSHV latency-associated nuclear antigen (LANA) dots, as detected by immunofluorescence microscopy, indicating a higher viral genome copy number. Cells in 3-D also demonstrated a higher rate of lytic reactivation. The 3-D microwell array system has the potential to improve 3-D cell oncology models and allow for better-controlled studies for drug discovery.
Source: Scientific Reports
DOI: 10.1038/srep39144
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,690
Funders: National Science Foundation, Brigham and Women's Hospital, National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Biomedical Imaging and Bioengineering, National Institute of Dental and Craniofacial Research
Awards: G1155640117, Novel disposable microchips for HIV-1 viral load, G3301064596, G3330864316, Portable Nanostructured Photonic Crystal Device for HIV-1 Viral Load, G4021249156, G4642296646, G5135944688, G5520316217, G5729131322, G6548553779, Single Cell Characterization of Latent HIV-1 Reservoirs, G9000022240, G9000045412, G9000099863, G9000112847, G9000134163, G9000213969, G953961477, CAREER: Non-invasive fields for directed 3D microgel assembly for tissue engineering, G985056232
OpenAlex ID: W2563947691