Work

Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases

Year: 2016

Type: review

Abstract: The development of customizable sequence-specific nucleases such as TALENs, ZFNs and the powerful CRISPR/Cas9 system has revolutionized the field of genome editing. The CRISPR/Cas9 system is particularly versatile and has been applied in numerous species representing all branches of life. Regardless of the target organism, all researchers using sequence-specific nucleases face similar challenges: confirmation of the desired on-target mutation and the detection of off-target events. Here, we evaluate the most widely-used methods for the detection of on-target and off-target mutations in terms of workflow, sensitivity, strengths and weaknesses.

Source: Biotechnology Advances

DOI: 10.1016/j.biotechadv.2016.12.003

Authors: Julia Zischewski, Rainer Fischer, Luisa Bortesi

Institutions: RWTH Aachen University, Fraunhofer Institute for Molecular Biology and Applied Ecology

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $5,460

Funders: European Research Council, FP7 Ideas: European Research Council