Microfluidic platform combining droplets and magnetic tweezers: application to HER2 expression in cancer diagnosis
Year: 2016
Type: article
Abstract: The development of precision medicine, together with the multiplication of targeted therapies and associated molecular biomarkers, call for major progress in genetic analysis methods, allowing increased multiplexing and the implementation of more complex decision trees, without cost increase or loss of robustness. We present a platform combining droplet microfluidics and magnetic tweezers, performing RNA purification, reverse transcription and amplification in a fully automated and programmable way, in droplets of 250nL directly sampled from a microtiter-plate. This platform decreases sample consumption about 100 fold as compared to current robotized platforms and it reduces human manipulations and contamination risk. The platform's performance was first evaluated on cell lines, showing robust operation on RNA quantities corresponding to less than one cell, and then clinically validated with a cohort of 21 breast cancer samples, for the determination of their HER2 expression status, in a blind comparison with an established routine clinical analysis.
Source: Scientific Reports
DOI: 10.1038/srep25540
Institutions: Centre National de la Recherche Scientifique, Université Paris Sciences et Lettres, Institut Pierre-Gilles de Gennes pour la Microfluidique, Physique des Cellules et Cancers, Institut Curie, Inserm, Assistance Publique – Hôpitaux de Paris, Hôpital Saint-Louis, Université Paris Diderot, Laboratoire d'Analyse et d'Architecture des Systèmes, Université de Toulouse
Language: English
Subfield: Fluid Flow and Transfer Processes
Field: Chemical Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,690
OpenAlex ID: W2345566549