Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung
Year: 2013
Type: article
Abstract: The lung hosts multiple populations of macrophages and dendritic cells, which play a crucial role in lung pathology. The accurate identification and enumeration of these subsets are essential for understanding their role in lung pathology. Flow cytometry is a mainstream tool for studying the immune system. However, a systematic flow cytometric approach to identify subsets of macrophages and dendritic cells (DCs) accurately and consistently in the normal mouse lung has not been described. Here we developed a panel of surface markers and an analysis strategy that accurately identify all known populations of macrophages and DCs, and their precursors in the lung during steady-state conditions and bleomycin-induced injury. Using this panel, we assessed the polarization of lung macrophages during the course of bleomycin-induced lung injury. Alveolar macrophages expressed markers of alternatively activated macrophages during both acute and fibrotic phases of bleomycin-induced lung injury, whereas markers of classically activated macrophages were expressed only during the acute phase. Taken together, these data suggest that this flow cytometric panel is very helpful in identifying macrophage and DC populations and their state of activation in normal, injured, and fibrotic lungs.
Institution: Northwestern University
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funders: U.S. Department of Veterans Affairs, National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of Allergy and Infectious Diseases, National Institute of Environmental Health Sciences, National Institute of Arthritis and Musculoskeletal and Skin Diseases
Awards: G1187687215, Cardiovascular Regenerative Medicine, G1882761228, Therapeutic Role for P21 in Suppressing IL-1 Beta Mediated Pathologies, Development of a Novel RA/Atherosclerosis Mouse Model, Pathophysiology of Acute Lung Injury, A novel and improved mouse system for studying macrophage specific gene deletion, G5751941632, Signaling in the lung induced by particulate matter air pollution, Role of BH3-Domain Proteins in the Effector Phase of RA, Role of alveolar macrophages in particulate matter-induced cardiopulmonary disease, G9000222627, The Robert H. Lurie Comprehensive Cancer Center
OpenAlex ID: W2154434805