Conditional p53 Deletion Promotes Adult Neurogenesis and Improves the Acquisition and Clearance of Contextual Fear Memory
Year: 2014
Type: dissertation
Abstract: We crossed Nestin-CreERT2 mice and p53 flox mice to produce mice with heterozygous or homozygous p53 deletion in a tamoxifen (TAM)-dependent manner (named iKO-p53+/- and iKO-p53-/- mice, respectively). Result showed that dividing Ki-67+ cells and immature DCX+ neurons in the dentate gyrus were increased in iKO-p53+/- and iKO-p53-/- mice 4 weeks after the TAM injection. To study the retrograde effect of p53 deletion on behaviors, mice received TAM after the training in the CFC and was tested 4 week later. iKO-p53+/- mice displayed degraded contextual fear memory. To study the anterograde effect of p53 deletion on behaviors, mice received TAM 8 weeks before the training in the CFC and was tested o1 day after the training. Results showed a trend of increased contextual fear memory. Therefore the result provide evidence that increased adult neurogenesis is associated with improved acquisition and clearance of hippocampus-dependent contextual fear memory.
Source: TSpace (University of Toronto)
Author: Xiaochen Hu
Language: English
Subfield: Developmental Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2567490820