Work

Understanding development and stem cells using single cell-based analyses of gene expression

Year: 2017

Type: article

Abstract: In recent years, genome-wide profiling approaches have begun to uncover the molecular programs that drive developmental processes. In particular, technical advances that enable genome-wide profiling of thousands of individual cells have provided the tantalizing prospect of cataloging cell type diversity and developmental dynamics in a quantitative and comprehensive manner. Here, we review how single-cell RNA sequencing has provided key insights into mammalian developmental and stem cell biology, emphasizing the analytical approaches that are specific to studying gene expression in single cells.

Source: Development

DOI: 10.1242/dev.133058

Authors: Pavithra Kumar, Yuqi Tan, Patrick Cahan

Institutions: Johns Hopkins University, Johns Hopkins Medicine

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases

Award: G2443268587