Affinity Release of Insulin-like Growth Factor-1 Increases the Viability of Retinal Pigment Epithelium Cells In Vitro
Year: 2015
Type: dissertation
Abstract: Poor cell survival in vitro and in vivo is a key challenge in tissue engineering. Pro-survival therapeutic proteins such as insulin-like growth factor-1 (IGF-1) can promote cell viability but require controlled delivery systems due to their brief half-lives. Biocompatible materials are commonly used for drug-delivery platforms or to encapsulate cells for increased viability but no material exists for simultaneous use in both applications. A blend of hyaluronan and methylcellulose known to promote cell survival was modified with Src homology 3 (SH3)-binding peptides and demonstrated tunable, affinity-based release of the pro-survival fusion protein SH3-IGF-1. The material was also shown to significantly increase the viability of retinal pigment epithelium cells (RPE) in anchorage-independent conditions. This novel system is applicable to a broad range of cells and protein therapeutics and is a promising strategy for a drug-delivery/cell transplantation strategy to increase the viability of both exogenous and endogenous cells in tissue engineering applications.
Source: TSpace (University of Toronto)
Author: James Parker
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2568172507