Hydrodynamic Force-transduced Cyotskeletal Remodeling Stabilizes Leukocyte Integrin-mediated Adhesions
Year: 2016
Type: dissertation
Abstract: The emigration of monocytes from the blood into tissues is a fundamental component of inflammation. Monocytes roll and arrest on inflamed endothelium and stably adherent cells are subjected to a hydrodynamic force imparted by flowing blood. We used parallel plate flow chamber assays and confocal imaging to investigate actin cytoskeleton dynamics in the stabilization of integrin-mediated adhesion. The ability of U937 cells to resist detachment and flow-induced cell deformation when adhered to surfaces coated with VCAM-1 and ICAM-1 was dependent on actin polymerization. Hydrodynamic force triggered cytoskeletal remodeling, cell spreading and increased contact area with the adhesion surface. This force-induced signaling also occurredin the absence of integrin ligands. Our data suggest that hydrodynamic force induces integrin-dependent and -independent signaling that contribute to cytoskeletal remodeling, strengthening of cell adhesion and resistance to deformation whichenable U937 cells to stabilize their adhesion in the presence of hydrodynamic force.
Source: TSpace (University of Toronto)
Author: Hisham Ibrahim
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2570929582