Year: 2017
Type: article
Abstract: The retinal degeneration 11 ( rd11 ) mouse is a new animal model with rapid photoreceptor degeneration. The long-term efficacy of gene therapy has a direct relationship with the onset of photoreceptor degeneration or apoptosis, whereas the degeneration or apoptosis patterns of photoreceptors are still unclear in rd11 mice. The distribution patterns of cone function-related L- and S-opsin were examined by immunofluorescence staining, and the apoptosis was performed by TUNEL assay in rd11 mice. The expression pattern of L-opsin or S-opsin in rd11 retina at postnatal day (P) 14 was similar to the pattern observed in wildtype retina. With increasing age, the expression of L-opsin and S-opsin, especially S-opsin, decreased significantly in rd11 mice. The degeneration of L-opsin began around the optic nerve and expanded to the periphery of the retina, from the ventral/nasal to dorsal/temporal retina, whereas the expression of S-opsin gradually decreased from the dorsal/temporal to ventral/nasal retina. Apoptotic signal appeared at P14 and was strongest at P28 of rd11 mice. The key genes associated with apoptosis confirmed those changes. These indicated that the degeneration and apoptosis of cone photoreceptors began at P14 of rd11 mice, which was a key point for gene therapy.
Source: Journal of Ophthalmology
DOI: 10.1155/2017/9721362
Authors: Hua Zhang, Xia Li, Xufeng Dai, Juanjuan Han, Yangyang Zhang, Yan Qi, Ying He, Yan Liu, Bo Chang, Ji-jing Pang
Institutions: Wenzhou Medical University, Affiliated Eye Hospital of Wenzhou Medical College, First Affiliated Hospital of GuangXi Medical University, Shanxi Medical University, Shanxi University of Medicine, Shandong Eye Institute & Hospital, Shandong First Medical University, Jackson Laboratory, University of Florida
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,040
Awards: G1090198237, G1725898606, G1980898700, G2008207429, G2170639031, G3083737768, G3117195592, G3268092671, G3422681555, G3988243532, G4096229155, G4199635355, G4424134958, G452591597, G4637648831, G4841900080, G4882438780, G5282009331, G552440617, Vitreal delivery of novel AAV vectors for CNGA3 achromatopsia cone gene therapy, G5555528532, G5557944849, G6156583721, G6259575582, G6276959314, G6295988520, G6462478929, G6637348191, G6728452291, G6785625244, G6828068979, G6922443858, G6998412468, Eye Mutant Resource, G7347773102, G7456711194, G7814919367, G7865416066, G8152274505, G8708396292, 多点突变AAV2载体玻璃体腔注射转导猴视网膜色素上皮细胞的研究, G8886248709, G926211759
OpenAlex ID: W2572443831