Year: 2017
Type: article
Abstract: The intrinsically disordered scaffold proteins AFF1/4 and the transcription elongation factors ELL1/2 are core components of the super elongation complex required for HIV-1 proviral transcription. Here we report the 2.0-Å resolution crystal structure of the human ELL2 C-terminal domain bound to its 50-residue binding site on AFF4, the ELLBow. The ELL2 domain has the same arch-shaped fold as the tight junction protein occludin. The ELLBow consists of an N-terminal helix followed by an extended hairpin that we refer to as the elbow joint, and occupies most of the concave surface of ELL2. This surface is important for the ability of ELL2 to promote HIV-1 Tat-mediated proviral transcription. The AFF4-ELL2 interface is imperfectly packed, leaving a cavity suggestive of a potential binding site for transcription-promoting small molecules.
Source: Nature Communications
DOI: 10.1038/ncomms14076
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Funders: U.S. Department of Energy, Sandler Foundation, National Natural Science Foundation of China, National Institutes of Health, Office of Science, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, Basic Energy Sciences, Biological and Environmental Research, Lawrence Berkeley National Laboratory
Awards: G1079818582, G1143651532, G1229526393, G1286236842, G1286251239, G1337544421, G1414775356, G1445641779, G1581387393, G1785801686, G1804828992, G1816284028, G1831385526, G1939440326, G223002938, G2300450775, G2338633307, G2371548206, G2857863752, G303061748, G30714459, G3083819904, G315621713, G3295861670, G3432487278, G3488436650, G3629260272, G365575497, G3657500132, G382641700, G3884951186, G419881185, G42916606, G4501827968, G495301720, G498139845, Minstrel HTUV Gallery 700 Automated Crystal Growth and Imaging System, G5570207115, G5849692847, G628751139, G6327353172, G650090426, G654302390, G6550344761, G6652958823, G704498496, G7283024673, G7354628648, G7787287669, A Synchroton Radiation Structural Biology Resource, G805243471, G8382000481, G8925327636, G9000029007, G9000069621, G9000145002, G9000253465, G911061179, G969889393
OpenAlex ID: W2572893218