Negative Purification Method for the Selection of Specific Antibodies from Polyclonal Antisera
Year: 2002
Type: article
Abstract: We developed a protocol to remove non-specific antibodies from polyclonal antisera by adsorption on non-target antigens immobilized on nitrocellulose membranes. This "negative" purification method is simple and provides better immunoreagents than the blocking of nonspecific antibodies in solution or the enrichment of specific antibodies on nitrocellulose membranes. For routine applications, this method is quicker and cheaper than the purification protocols based on selective precipitations and affinity chromatography.
Source: BioTechniques
DOI: 10.2144/02335st06
Authors: Cindy Benet, Pierre Van Cutsem
Institution: University of Namur
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,415
OpenAlex ID: W92386518