Morphological and molecular identification and fungicide sensitivity assay of pathogens attacking guyabano (Annona muricata) in Philippines
Year: 2016
Type: article
Abstract: This study characterizes the plant pathogens attacking guyabano fruits and leaves through morphological and molecular approaches and determines their sensitivity to fungicides.Three fungal pathogens were found on guyabano fruit, Colletotrichum gloeosporioides, C. acutatum and Fusarium chlamydosporum.Colletotrichum gloeosporioides was also found to infect the leaves.Molecular identification of the Colletotrichum species was carried out through amplification of rDNA ITS regions by using species specific primers: CgInt for C. gloeosporioides and CaInt2 for C. acutatum in combination with ITS4 universal primer.Ver ITS primer was used on Fusarium species.All fungal pathogens were found to be highly pathogenic to guyabano fruits and leaves.Sensitivity assay revealed that all three pathogens were highly susceptible to captan, tebuconazole and difenoconazole + propiconazole.
Source: Plant Pathology & Quarantine
DOI: 10.5943/ppq/6/1/9
Author: R. T. Alberto
Institution: Central Luzon State University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2580085390