Additional file 1: Figure S1. of Surface attachment, promoted by the actomyosin system of Toxoplasma gondii is important for efficient gliding motility and invasion
Year: 2017
Type: other
Abstract: Investigating various actin antibodies for specificity. The specificity of four antibodies raised against TgACT1 and two against PfACT1 were compared using western blotting and immunofluorescence assay (IFA). Commercially available actin antibodies were also tested; anti-β-actin (Sigma) and ACTN05(C4) (Abcam). (A) Immunoblot analysis is evaluating the specificity of the antibodies to actin from either host (HFF) or Toxoplasma cell lysates. All apicomplexan antibodies are specific to Toxoplasma actin whereas the commercial antibodies label both HFF and parasite actin at 42 kDa. (B) IFA of act1 cKO parasites at 4 days after induction. (C) IFA analysis of act1 cKO parasites at 8 days after induction. Some antibodies show persistent signal that is still present up to 8 days post-induction, when western blot or IFA with other antibodies show actin has dropped below detectable levels. Scale bars: 10 μm. (TIF 1787 kb)
Source: Figshare
Authors: Whitelaw, Jamie, Latorre-Barragan, Fernanda, Gras, Simon, Gurman Pall, Leung, Jacqueline, Heaslip, Aoife, Egarter, Saskia, Andenmatten, Nicole, Nelson, Shane, Warshaw, David, Ward, Gary, Meissner, Markus
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2581421696