Work

The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies

Year: 2017

Type: review

Abstract: High throughput genomic assays empower us to study the entire human genome in short time with reasonable cost. Formalin fixed-paraffin-embedded (FFPE) tissue processing remains the most economical approach for longitudinal tissue specimen storage. Therefore, the ability to apply high throughput genomic applications to FFPE specimens can expand clinical assays and discovery. Many studies have measured the accuracy and repeatability of data generated from FFPE specimens using high throughput genomic assays. Together, these studies demonstrate feasibility and provide crucial guidance for future studies using FFPE specimens. Here, we summarize the findings of these studies and discuss the limitations of high throughput data generated from FFPE specimens across several platforms that include microarray, high throughput sequencing, and NanoString.

Source: International Journal of Genomics

DOI: 10.1155/2017/1926304

Authors: Pan Zhang, Brian D. Lehmann, Yu Shyr, Yan Guo

Institution: Vanderbilt University

Language: English

Topic: Gene expression and cancer classification

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,380