563 Influence of Ultra-high Levels of Carbon Dioxide on Secondary Metabolite Production in Vitro
Year: 1999
Type: article
Abstract: The influence of a wide range of CO 2 levels on the growth, morphogenesis, and secondary metabolite production in vitro was evaluated. Shoots of thyme ( Thymus vulgaris L.) and a spearmint–peppermint cross ( Mentha spicata × Mentha piperita ) were grown on MS medium with and without 3% sucrose under 350, 1500, 3000, 10,000, and 30,000 μL CO 2 /L for 8 weeks. Dichloromethane extracts from leafs were analyzed using GC-MS techniques. Prominent peaks were identified by comparison with known standards. Highest growth (i.e., fresh weight) and morphogenesis responses (i.e., leafs, shoots and roots) were obtained when shoots were grown under 10,000 μL CO 2 /L regardless of whether or not sucrose was included in the medium. Ultra-high CO 2 concentrations (3000 μL CO 2 /L) stimulated secondary metabolite production regardless of whether or not the medium contained sucrose. However, the combination of certain ultra-high CO 2 levels (e.g., 3000 to 10,000 μL CO 2 /L) and the presence of sucrose in the medium resulted in shoots producing the highest levels of secondary metabolites. These results suggest that in vitro photosynthesis, which is stimulated by ultrahigh CO 2 levels, may enhance secondary metabolite production.
Source: HortScience
Authors: Brent Tisserat, Steven Vaughn
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,000
OpenAlex ID: W2582046599