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Evaluation of a research use only luminex based assay for measurement of procalcitonin in serum samples.

Year: 2016

Type: article

Abstract: Human Acute Phase Multiplex Assay, Bio-Rad Laboratories, Hercules, CA) for comparability with an FDA cleared assay for procalcitonin (VIDAS B.R.A.H.M.S. PCT Assay, bioMérieux, Durham, NC). We tested 1,072 serum samples collected from patients with suspected sepsis in an intensive care unit setting for the comparison. There was poor correlation of the luminex based assay (r=0.081) with the VIDAS PCT Assay in the clinically relevant measurement range (<10 ng/mL). Additionally the Bio-Plex assay showed poor precision. Mass-spectrometry analysis of material eluted from PCT beads did not reveal any identifiable procalcitonin. The results show that research use only assays need to be validated to determine their suitability for research studies.

Source: PubMed Central

Authors: Charles Garrigan, Jennifer Han, Pam Tolomeo, Katherine J Johnson, Stephen R Master, Ebbing Lautenbach, Irving Nachamkin

Institutions: University of Pennsylvania, Fox Chase Cancer Center, Weill Cornell Medicine

Language: English

Topic: Sepsis Diagnosis and Treatment

Subfield: Critical Care and Intensive Care Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2582175001

FWCI:

Cites: 14

Cited by: 2

Related to: 10