Work

Prion Transfection of Yeast

Year: 2017

Type: preprint

Abstract: Transfection of yeast with amyloid filaments, made from recombinant protein or prepared from extracts of cells infected with a prion, has become an important method in characterizing yeast prions. Here, we describe a method for transmission of [URE3] with Ure2p amyloid that is based on a previously published protocol for transfection with Sup35p filaments to make cells [PSI+]. This method may be used for other prions by changing just the amyloid source, host strain, and plating medium.

Source: Cold Spring Harbor Protocols

DOI: 10.1101/pdb.prot089037

Authors: Herman K. Edskes, Dmitry Kryndushkin, Frank Shewmaker, Reed B. Wickner

Institutions: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases, Uniformed Services University of the Health Sciences

Language: English

Topic: Prion Diseases and Protein Misfolding

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases

OpenAlex ID: W2583742359

Cites: 14

Cited by: 4

Related to: 10