Year: 1990
Type: article
Abstract: Friable callus from leaf disks of Rosa hybrida `Tiffany' was initiated within two weeks under dark conditions and 25°C on Murashige and Skoog (MS) medium supplemented with 4 mg.liter -1 2,4-D. Callus was then transferred into MS medium containing 3 mg.liter -1 2,4-D. Within four weeks, rhizogenesis occurred on the callus surface. The rhizogenic calllus was subculture on MS medium plus 3 mg.liter -1 2,4-D every 4-6 weeks. Within six months from initial culture, somatic embryos were developed on the aging callus in darkness. Transfer of the aging callus with somatic embryos into 1/2 MS medium containing 1 mg.liter -1 kinetin and maintaining it under 46 μE m -2 s -1 light for 16 hrs. resulted in greening of the somatic embryos.
Source: HortScience
Authors: Mahmoud B. Arif, Houchang Khatamian
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2587621631