Changes in IL-4 and IL-13 expression in allergic-rhinitis treated with hydrogen-rich saline in guinea-pig model
Year: 2017
Type: article
Abstract: Background Medical gas hydrogen (H 2 ) has a special role in airway inflammation ; however, the effect of H 2 on allergic rhinitis (AR) remains unclear. This study explored the possible roles of H2 on the pathogenesis of AR and observed the influences of H 2 on cytokines IL-4 and IL-13. Methods An AR guinea pig model was established by nasal ovalbumin sensitisation. Eighteen guinea pigs were divided into three groups, namely, saline control, AR-sensitised, and hydrogen-rich saline (HRS)-treated groups, with each group having six guinea pigs. The frequencies of sneezing and scratching were recorded. The IgE level and cytokine (IL-4 and IL-13) levels in the serum were measured. The expression levels of IL-4 and IL-13 mRNA and protein in the nasal mucosa were also determined by real-time reverse transcriptase–polymerase chain reaction and Western blot . We also observed the infiltration of cytokine (IL-4 and IL-13) in nasal mucosa by immunofluorescence. Results The frequencies of sneezing and scratching, as well as the levels of IgE, IL-4, and IL-13, in the serum were higher in the AR group than in the control group ( p < 0.01), whereas all these parameters were decreased significantly after HRS treatment ( p < 0.05). The expression levels of IL-4 and IL-13 mRNA and protein in the nasal mucosa were also lower in guinea pigs treated with HRS than those in the AR group ( p < 0.05). Conclusions HRS could affect anti-inflammation in AR and decreased the expression of IL-4 and IL-13.
Source: Allergologia et Immunopathologia
Institution: Tongji University
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,000
OpenAlex ID: W2588311770