Work

Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis

Year: 2017

Type: article

Abstract: In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.

Source: Stem Cell Reports

DOI: 10.1016/j.stemcr.2017.01.010

Authors: Julia TCW, Claudia M.B. Carvalho, Bo Yuan, Shen Gu, Alyssa N. Altheimer, Shane McCarthy, Dheeraj Malhotra, Jonathan Sebat, Arthur J. Siegel, Uwe Rudolph, James R. Lupski, Deborah L. Levy, Kristen J. Brennand

Institutions: Icahn School of Medicine at Mount Sinai, Baylor College of Medicine, Cold Spring Harbor Laboratory, F. Hoffmann-La Roche AG, University of California San Diego, Harvard University, McLean Hospital, Texas Children's Hospital

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $4,190

Funders: Ellison Medical Foundation, New York Stem Cell Foundation, National Institutes of Health, National Institute of Mental Health, National Institute of Neurological Disorders and Stroke

Awards: G1602558761, G2768437882, G4642494767, Neurobiology of a Mutation in Glycine Metabolism in Psychotic Disorders, G5890084902, Contrasting causal microRNAs in forebrain and midbrain COS hiPSC neural cells, G9000015070, G9000097117, G9000248693, G9000291259

OpenAlex ID: W2588409393

FWCI: 0.67

Cites: 32

Cited by: 11

Related to: 10