Sp061transient Increase in Cd39 Ameliorates Acute Renal Injury Whereas Chronic Increase in Cd39 Exacerbates Renal Fibrosis After Ischemia Reperfusion Injury: Evidence for a Profibrotic Action of Chronically Elevated Adenosine Level
Year: 2015
Type: conference-abstract
Abstract: Introduction and Aims: Ischemia reperfusion injury (IRI) is a common cause of acute kidney injury and increases the risk of chronic kidney disease (CKD), which is characterised by renal fibrosis. Increased CD39 (apyrase) levels protect the kidney from acute IRI. However, the impact of increased CD39 levels on the longer-term outcome from IRI is not known. Aim: To examine the impact of increased CD39 activity on the acute and chronic phases of IRI Methods: We examined both acute and chronic effects of unilateral renal IRI (23.5 min ischemia) in two models of increased renal CD39 levels: mice that overexpress human CD39 (CD39Tg) and mice administered soluble apyrase by intraperitoneal injection. Renal nucleotide levels were measured at baseline, after 23.5 min ischemia, and at 24 hours and 4 weeks post-IRI using high performance liquid chromatography. At 24 hours post-IRI, renal injury was assessed using serum creatinine (Cr) and tubular injury score. At 4 weeks post-IRI, renal fibrosis was assessed using Masson’s trichrome staining and morphometric scoring with the Aperio ScanScope.
Institutions: The University of Melbourne, St Vincent's Hospital Melbourne
Language: English
Topic: Acute Kidney Injury Research
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2588594028