Work

Engineered holocytochrome c synthases that biosynthesize new cytochromes c

Year: 2017

Type: article

Abstract: Significance Specific mutations in human holocytochrome c synthase (HCCS) confer enhanced biosynthesis of cyt c , including cyt c variants never before produced. These include a bis-Met and single thioether cyt c . We designate these as HCCS “release mutants” because evidence indicates they are mechanistically affected in step 4 of biosynthesis, release of cyt c from the HCCS/cyt c complex. Characteristics of cyt c WT (His/Met), cyt c H19M (bis-Met), cyt c M81H (bis-His), and cyt c M81A (His/OH − ) indicate proper folding and that the engineered ligands are present. This panel of variants spans a redox gradient of more than 400 mV, which will be useful for studying various redox-dependent cellular processes and other physical properties.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.1615929114