Work

Micro- and nanocrystal preparation for MicroED and XFEL serial crystallography by fragmentation of imperfect crystals

Year: 2017

Type: article

Abstract: This protocol describes different techniques to fragment protein crystals for high-resolution structure determination by MicroED and serial crystallography at an X-ray free electron laser.These techniques involve agitation of the crystal of interest: 1) by vigorous pipetting, 2) by sonication, and 3) by vortexing.Aside from setup, the time needed to perform these steps ranges from seconds to minutes.

Source: Protocol Exchange

DOI: 10.1038/protex.2017.010

Authors: M. Jason de la Cruz, M. Jason de la Cruz, Johan Hattne, Dan Shi, Jose A. Rodriguez, Francis E. Reyes, Tamir Gonen

Institutions: Howard Hughes Medical Institute, Janelia Research Campus, University of California, Los Angeles

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

OpenAlex ID: W2588970975

FWCI: 0.11

Cites:

Cited by: 2

Related to: 10