Tools for Studying Glycans: Recent Advances in Chemoenzymatic Glycan Labeling
Year: 2017
Type: review
Abstract: The study of cellular glycosylation presents many challenges due, in large part, to the nontemplated nature of glycan biosynthesis and glycans' structural complexity. Chemoenzymatic glycan labeling (CeGL) has emerged as a new technique to address the limitations of existing methods for glycan detection. CeGL combines glycosyltransferases and unnatural nucleotide sugar donors equipped with a bioorthogonal chemical tag to directly label specific glycan acceptor substrates in situ within biological samples. This article reviews the current CeGL strategies that are available to characterize cell-surface and intracellular glycans. Applications include imaging glycan expression status in live cells and tissue samples, proteomic analysis of glycoproteins, and target validation. Combined with genetic and biochemical tools, CeGL provides new opportunities to elucidate the functional roles of glycans in human health and disease.
Source: ACS Chemical Biology
Institutions: Scripps Research Institute, Vassar College
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: Chemoenzymatic methods for the detection of cellsurface glycans, G1735324999, Role of Siglec-10/G as a tumor suppressor in B-cells, Chemical tools for studying membrane protein glycosylation, G3299835760, G5503699249, Chemical Tools for Studying Fucosylated Glycans, Inducing antigen specific B cell tolerance, Regulation of Sialoside Expression in Leukocytes
OpenAlex ID: W2589017504