Year: 2016
Type: article
Abstract: VOLUME 284 (2009) PAGES 11309–11317 In this article, we provided immunological, histological, and physiological evidence that hyaluronan mediates the development of inflammation and airway hyperresponsiveness (AHR) after ozone inhalation (1Garantziotis S. Li Z. Potts E.N. Kimata K. Zhuo L. Morgan D.L. Savani R.C. Noble P.W. Foster W.M. Schwartz D.A. Hollingsworth J.W. Hyaluronan mediates ozone-induced airway hyperresponsiveness in mice.J. Biol. Chem. 2009; 284: 11309-11317Abstract Full Text Full Text PDF PubMed Scopus (103) Google Scholar). After publication of our article, we became aware that the airway physiology measurements reported in Figs. 5, 6, and 9 of the original manuscript, which were provided by the animal pulmonary physiology laboratory at Duke University, may have been unreliable.FIGURE 6(replication of original fig. 9D). Instillation of HMW-HA before and after ozone exposure does not significantly ameliorate AHR, although a trend is observed (n = 9–10 mice per group).View Large Image Figure ViewerDownload Hi-res image Download (PPT) We therefore had two separate laboratories, one at Duke University under the supervision of Dr. Herman Staats, and the other at the National Institute of Environmental Health Sciences under the supervision of Dr. Stavros Garantziotis, repeat the experiments in question. These laboratories have replicated the key observation that hyaluronan mediates ozone-induced airway hyperresponsiveness. We again demonstrated that CD44 and inter-α-inhibitor (IαI) are necessary for the development of ozone-induced AHR (FIGURE 1, FIGURE 2, n = 5–10 per group; p < 0.05). Furthermore, we confirmed that hyaluronan-binding protein (HABP) decreases AHR after ozone exposure (Fig. 3, n = 4–5 per group; p < 0.05). We showed that short fragment hyaluronan (sHA; molecular mass of 100–400 kDa) but not vehicle or high molecular weight hyaluronan (HMW-HA; molecular mass > 1,000 kDa) induces AHR in naïve C57BL/6 mice (Fig. 4, n = 4–6 mice per group; p < 0.05), but not in CD44-deficient mice (Fig. 5, n = 4–8 mice per group; p < 0.05). We were unable to confirm that HMW-HA ameliorates ozone-induced AHR, although there was a trend to that direction (Fig. 6, n = 8–10 mice per group; p = 0.04 but not significant by Holm-Sidak analysis and α = 5%).FIGURE 1(replication of original Fig. 5A). IαI is essential for the development of ozone-induced airway hyperresponsiveness (n = 7–8 per group). *, p < 0.05; **, p < 0.01 compared to IaI−/− ozone.View Large Image Figure ViewerDownload Hi-res image Download (PPT)FIGURE 3(replication of original Fig. 6C). Hyaluronan-binding protein (HABP), but not scrambled protein (SBP), significantly decreased AHR after ozone exposure (n = 4–5 per group). *, p < 0.05.View Large Image Figure ViewerDownload Hi-res image Download (PPT)FIGURE 4(replication of original fig. 9A). sHA but not HMW-HA or vehicle induces AHR in naïve C57BL/6 mice (n = 4–6 mice per group; p < 0.05 sHA versus HMW-HA).View Large Image Figure ViewerDownload Hi-res image Download (PPT) In conclusion, we report that the repeated work largely validates the originally reported findings. Of note, the major conclusion of this article, i.e., that sHA mediates AHR after oxidative lung injury, has been independently validated in studies performed since this publication. For example, sHA mediates AHR after chlorine gas exposure, whereas HMW-HA or IαI blockade inhibits chlorine-induced AHR (2Lazrak A. Creighton J. Yu Z. Komarova S. Doran S.F. Aggarwal S. Emala Sr., C.W. Stober V.P. Trempus C.S. Garantziotis S. Matalon S. Hyaluronan mediates airway hyperresponsiveness in oxidative lung injury.Am. J. Physiol. Lung Cell. Mol. Physiol. 2015; 308: L891-L903Crossref PubMed Scopus (45) Google Scholar). Furthermore, exposure to ozone has been independently shown to result in increased levels of hyaluronan in the bronchoalveolar lavage fluid of mice (3Williams A.S. Mathews J.A. Kasahara D.I. Wurmbrand A.P. Chen L. Shore S.A. Innate and ozone-induced airway hyperresponsiveness in obese mice: role of TNF-α.Am. J. Physiol. Lung Cell. Mol. Physiol. 2015; 308: L1168-L1177Crossref PubMed Scopus (27) Google Scholar) and humans (4Bauer R.N. Müller L. Brighton L.E. Duncan K.E. Jaspers I. Interaction with epithelial cells modifies airway macrophage response to ozone.Am. J. Respir. Cell Mol. Biol. 2015; 52: 285-294Crossref PubMed Scopus (27) Google Scholar, 5Hernandez M.L. Lay J.C. Harris B. Esther Jr., C.R. Brickey W.J. Bromberg P.A. Diaz-Sanchez D. Devlin R.B. Kleeberger S.R. Alexis N.E. Peden D.B. Atopic asthmatic subjects but not atopic subjects without asthma have enhanced inflammatory response to ozone.J. Allergy Clin. Immunol. 2010; 126: 537-544.e1Abstract Full Text Full Text PDF PubMed Scopus (58) Google Scholar). Exposure to ozone was independently shown to fragment hyaluronan from HMW-HA to sHA (6Wu Y. Preparation of low-molecular-weight hyaluronic acid by ozone treatment.Carbohydr. Polym. 2012; 89: 709-712Crossref PubMed Scopus (62) Google Scholar). Furthermore, studies of murine asthma show that TSG-6, which links hyaluronan to IαI, is necessary for the development of inflammatory AHR (7Swaidani S. Cheng G. Lauer M.E. Sharma M. Mikecz K. Hascall V.C. Aronica M.A. TSG-6 protein is crucial for the development of pulmonary hyaluronan deposition, eosinophilia, and airway hyperresponsiveness in a murine model of asthma.J. Biol. Chem. 2013; 288: 412-422Abstract Full Text Full Text PDF PubMed Scopus (48) Google Scholar). Finally, CD44 is necessary for the development of sHA-induced AHR in a tracheal ring model (2Lazrak A. Creighton J. Yu Z. Komarova S. Doran S.F. Aggarwal S. Emala Sr., C.W. Stober V.P. Trempus C.S. Garantziotis S. Matalon S. Hyaluronan mediates airway hyperresponsiveness in oxidative lung injury.Am. J. Physiol. Lung Cell. Mol. Physiol. 2015; 308: L891-L903Crossref PubMed Scopus (45) Google Scholar). Together, these studies support that several models of pulmonary inflammation result in increased levels of sHA, which mediates AHR. In aggregate, our repeated studies confirm the original conclusions of the manuscript, and independent research also supports our originally reported conclusions. We therefore believe that our original report is accurate and valid.
Source: Journal of Biological Chemistry
Language: English
Subfield: Physiology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W2591132266