Year: 2017
Type: article
Abstract: MicroRNAs (miRNAs) are thought to exert their functions by modulating the expression of hundreds of target genes and each to a small degree, but it remains unclear how small changes in hundreds of target genes are translated into the specific function of a miRNA. Here, we conducted an integrated analysis of transcriptome and translatome of primary B cells from mutant mice expressing miR-17~92 at three different levels to address this issue. We found that target genes exhibit differential sensitivity to miRNA suppression and that only a small fraction of target genes are actually suppressed by a given concentration of miRNA under physiological conditions. Transgenic expression and deletion of the same miRNA gene regulate largely distinct sets of target genes. miR-17~92 controls target gene expression mainly through translational repression and 5'UTR plays an important role in regulating target gene sensitivity to miRNA suppression. These findings provide molecular insights into a model in which miRNAs exert their specific functions through a small number of key target genes.
Source: PLoS Genetics
Authors: Hyun Yong Jin, Hiroyo Oda, Pengda Chen, Chao Yang, Xiaojuan Zhou, Seung Goo Kang, Elizabeth Valentine, Jennifer M. Kefauver, Lujian Liao, Yaoyang Zhang, Alicia Gonzalez-Martin, Jovan Shepherd, Gareth J. Morgan, Tony S. Mondala, Steven R. Head, Pyeung-Hyeun Kim, Nengming Xiao, Guo Fu, Wen-Hsien Liu, Jiahuai Han, James R. Williamson, Changchun Xiao
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,043
Funders: Pew Charitable Trusts, Cancer Research Institute, National Research Foundation, National Natural Science Foundation of China, Chinese Academy of Sciences, Kangwon National University, National Research Foundation of Korea, Thailand Science Research and Innovation, National Institutes of Health, University of California, San Diego, University of California, San Francisco, Fundamental Research Funds for the Central Universities, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases
Awards: Regulation of T Follicular Helper Cell Differentiation by MicroRNAs, Assembly of E. coli ribosomes in vitro and in cells, G302678140, Deep Sequencing of Small Regulatory RNAs in Diffuse Large B cell Lymphoma, The Role of miR-17~92 in Autoimmune Diseases, Regulation of T Follicular Helper Cell Differentiation by MicroRNAs, G4946240625, Regulation of T Follicular Helper Cell Differentiation by MicroRNAs, G5609943137, Functional Analysis of MicroRNAs in Lymphocyte Development and Immune Tolerance, G6040768264, G614932463, G6272770403, G7048388025, G7362015272, G978477420
OpenAlex ID: W2592485210